IL-6 (D5W4V) XP® Rabbit MAb (Mouse Specific)

February 01, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

IL-6 (D5W4V) XP® Rabbit mAb (Mouse Specific) #12912

Catalog Number:

12912

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:2500 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blotting

Results Summary

IL-6 proteins were detected in tissue lysates.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal

The Bad

None

The Bottom Line

Good antibody for mouse IL-6 detection.

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