Mouse IL-4 Antibody

January 31, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse IL-4 Antibody

Catalog Number:

MAB404-100

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue homogenates

Primary Incubation

1 µg/m at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:2500 at room temperature for 2 hour

Tertiary Incubation

None

Detection

Western Blotting

Results Summary

Detected IL-4 proteins from tissue lysates.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signal

The Bad

None

The Bottom Line

Good antibody for IL-4 detection. Need to load high amounts of protein per well in gels.

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