Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb

January 25, 2017

Harvard Medical School
Harvard
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb

Catalog Number:

4377

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine ex-vivo immune cell assay lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation in 5% BSA

Blocking Agent

5% skimmed milk in 1X TBST

Secondary Incubation

1:3000 at room temperature for 2 hour in 5% skimmed milk

Tertiary Incubation

NA

Detection

Western Blotting

Results Summary

High levels of Phospho-p44/42 MAPK proteins were detected by western blotting.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good primary antibody, no background

The Bad

None

The Bottom Line

Good antibody for phospho ERK 1/2 detection, highly sensitive and minimum background.

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