eBioscience
Human CD45 PE conjugate
12-9459, clone 2D1
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The general aim of my research is to study acute myeloid leukemia. We are transplanting human leukemic cells into immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice and monitoring leukemic progression.
Flow Cytometry
Bone marrow isolated from NSG mice transplanted with human leukemic cells
1:100
Fc block is added to cells prior to staining with antibody
None
Flow cytometry on a BD LSRII-yellow machine
This antibody produces strong, reliable signal by flow cytometry. It provides good separation from mouse CD45 andibodies, allowing percentage chimerism in bone marrow of transplanted mice to easily be measured.
N/A
Strong signal, not a lot of antibody needed per test
Highly recommend!