Cajal Body Marker That Works Well In Human Cell Lines

January 11, 2017

Research
Aquinnah Pharmaceuticals
Scientist

Overall

Quality of Results

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Company:

Santa Cruz

Product Name:

Coilin Antibody (F-7)

Catalog Number:

sc-55594

Was using this antibody as part of a panel to look at localization of a protein within nuclear substructures. This was used in mouse, rat, and human cell lines. The mouse and rat cell lines displayed high background and diffuse non-specific staining, but the human cells showed distinct cajal bodies as puncta.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human, Mouse, and Rat Cell lines

Primary Incubation

1:200 in 5% Donkey Serum at 4C overnight

Blocking Agent

5% Donkey Serum RT 1hr

Secondary Incubation

1:400 Dylight Donkey anti-mouse

Tertiary Incubation

None

Detection

Microscopy

Results Summary

The antibody stained puncta assumed to be Cajal bodies in all cell lines tested. There was a large amount of diffuse (background?) staining present in the mouse and rat lines, while the human lines showed very bright and distinct puncta without any diffuse staining. The epitope is mapped to the human Coilin, so this may be the issue. Works great for human cells (H4 and HEK), but did not work as clearly for the mouse and rat lines (N2a and PC12).

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works well in human cell lines

The Bad

High background in non-human cell lines

The Bottom Line

This antibody is definitely worth a shot if you are looking to mark Cajal bodies in human cells/tissue. I wouldn't recommend this for staining non-human cells/tissues, as the high background is distracting.

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