Time Course Effect Of Chemoattractant On ABC1 Expression

January 13, 2017

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Santa Cruz Biotechnology

Product Name:

ABC1

Catalog Number:

sc-5491

ABC1 acts as a transporter which maintains the cholesterol efflux and maintains lipid homeostasis. Here we checked the time course effect of ABC1 by chemoattractant in macrophages.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:500 in 5% BSA prepared in 1x TBST

Blocking Agent

5% milk prepared in 1x TBST

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL solution

Results Summary

We found time dependent increase of ABC1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and the signal is prominent.

The Bad

None

The Bottom Line

Very good antibody to study the ABC1 expression by western blot.

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