Solid Antibody As Loading Control On Western Blot

Research
Aquinnah Pharmaceuticals
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-beta actin antibody

Catalog Number:

ab8226

This antibody consistently works well as a loading control for western blots. Also used as a positive control for the cytoplasmic fraction in subcellular fractionations.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human, Mouse, and Rat Cell Lysates and Rat Neuron Lysates

Primary Incubation

1:1000 in iBind FD Buffer

Blocking Agent

iBind FD Buffer for 5minutes

Secondary Incubation

1:10,000 Licor Fluorescent 680 anti-mouse

Tertiary Incubation

None

Detection

Licor Odyssey Fluorescent imager

Results Summary

Antibody works great as a loading control on Western blots. We have used it on both conventional overnight incubations as well as the Invitrogen iBind system. Signal is always strong. Also successfully used this as a positive control on western blots for subcellular fractionation.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good antibody as loading control

The Bad

None

The Bottom Line

Highly recommend for western blotting.

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