Anti-Human CD45RA for Naive T cells

December 21, 2016

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

APC Anti-Human CD45RA (HI100)

Catalog Number:

20-0458-T025

Anti-Human CD45RA APC was part of a multicolor panel used in a pediatric study involving healthy and asthmatic patients to compare Naive T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC from whole blood

Primary Incubation

PBMC were stained with an antibody cocktail containing CD45RA APC (clone HI100) dilution 1.25/100 for 15min at RT at dark

Blocking Agent

FcR block (Miltenyi), 3min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

PBMC were incubated with 2.5ul of FcR block for 3min, then PBMC were stained with an antibody cocktail containing CD45RA APC (clone HI100) dilution 1.25/100 for 15min at RT at dark in final volume of 50ul. Cells were washed twice with PBS + 2%FBS and acquired on LSRII. Good separation of Naive T cells CD45RA+ CXCR5- CD45RO-

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Nice separation, bright color, easy to titrate

The Bad

Donor dependent: the separation could smear a little, don't use too much antibody

The Bottom Line

Good separation of Naive T cells CD45RA+ CXCR5- CD45RO-

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