CD45RA V450 for Naive T cells

December 20, 2016

Immunology
The Jackson Laboratory for Genomic Medicine
Lab Manager, RA III

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Biosciences

Product Name:

violetFluor™ 450 Anti-Human CD45RA (HI100)

Catalog Number:

75-0458-T025

This antibody was used for sorting Naive T cells for RNA sequencing.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC from whole blood

Primary Incubation

PBMC were stained with an antibody cocktail containing CD45RA V450 (clone HI100) dilution 2.5/100 for 15min at RT at dark in total volume of 50ul

Blocking Agent

FcR block (Miltenyi), 3min

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII

Results Summary

Good separation of CD45RA+ T cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright, nice separation

The Bad

N/A

The Bottom Line

Good antibody

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