Embedding Samples For Cryopreservation

Boston University
Center for Regenerative Medicine
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fisher HealthCare

Product Name:

O.C.T.

Catalog Number:

23-730-571

The aim of this procedure was to cryopreserve cultured cells for downstream histological applications.

Experimental Design and Results Summary

Application

Embedding medium for frozen specimens

Starting Material

Cells or small frozen tissues

Protocol Overview

Add 1-1.5 ml of O.C.T medium to the frozen tissue during the cryopreservation protocol procedure. O.C.T is used as an embedding medium for frozen tissue.

Tips

Remove excess liquid or sucrose from the tissue prior to the addition of O.C.T.

Results Summary

O.C.T allows for optimal cutting temperature by providing a matrix for cryostat sectioning.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Easy cryostat sectioning of samples.

The Bad

Not applicable

The Bottom Line

Easy to use in the cryopreservation protocol.

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