Anti Human IFNgamma Antibody

December 05, 2016

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-human IFN-γ Antibody

Catalog Number:

502512

We use this antibody to stain human CD8 T cells following stimulation using various conditions to determine activation capacity of CD8 T cells isolated from different donors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD8 T cells

Primary Incubation

1:50, overnight, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry APC channel

Results Summary

We see a shift in interferon gamma production in cells stimulated for 12h with PMA and ionomycin. The antibody works well, minimal background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good staining

The Bad

None

The Bottom Line

Good antibody to use for intracellular staining of human CD8T cells

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