Antibody For Staining Of Newly Formed Perforin

December 01, 2016

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-human Perforin Antibody

Catalog Number:

353307

This antibody was used for intracellular staining of human CD8+ T cells upon 12h stimulation with PMA and ionomycin. The antibody works well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human CD8+ T cells

Primary Incubation

1:50, overnight, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry, PacificBlue channel

Results Summary

Staining is not dramatic but we do see a consistent upregulation in perforin in cells stimulated with PMA and ionomycin compared to cells left unstimulated (medium only).

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Consistent results

The Bad

Low intensity

The Bottom Line

Good antibody to use for intracellular staining of newly formed perforin.

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