Excellent Flow Cytometry Antibody For Human CD4

December 21, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Invitrogen, Thermo Fisher Scientific

Product Name:

CD4 Monoclonal Antibody (S3.5), Alexa Fluor 405

Catalog Number:

MHCD0426

I used to this antibody to stain CD4+ T-cells isolated from human PBMCs

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human T-cells

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I used to get nice distinct CD4+ staining which is of presentable quality

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

Worth buying it.

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