Good Antibody For CD69 Staining

December 08, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Anti-Mouse CD69 PE

Catalog Number:

12-0691-81

CD69 is considered as a early marker of T-cell activation. I use this antibody to measure activation of CD4+ T-cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:400 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I am getting good CD69 staining upon activation of T-cells stimulated with anti-CD3/CD28.

DOI or PMID #

NA

Additional Notes

Works with surface stainig only.

Related Categories

Image Gallery

Summary

The Good

Ease of optimization and reproducible results

The Bad

Somewhat weak staining

The Bottom Line

Worth buying it.

Share your experience with other scientists. Write a Review! »

Join the discussion