Best Phospho AKT Antibody For Flow Cytometry Analysis

November 21, 2016

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

eBioscience

Product Name:

Anti-Human/Mouse phospho-AKT (S473) PE

Catalog Number:

12-9715-41

I used to this antibody to study AKT phosphorylation in T-cells and antibody works fantastically.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse T-cells

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

0.5% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

I got distinct phosphorylated and non-phosphorylated populations of T-cells in the absence of TCR stimulation.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Little expensive

The Bottom Line

Worth buying

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