Use Of Acetylated alpha Tubulin Antibody From Abcam On Embryonic Tissue

November 21, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-Acetylated alpha Tubulin [6-11B-1] antibody

Catalog Number:

ab24610

This antibody was used to analyze the distribution of Acetylated alpha Tubulin and microtubules in embryonic tissues. This monoclonal anti Acetylated alpha Tubulin (clone 6-11B-1) antibody appears specific to microtubule, mainly in epithelial tissues.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Chicken embryonic epithelial tissue

Primary Incubation

1:200, overnight, at 4C in Blocking agent, on slide

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-488-conjugated anti-mouse IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on shaker

Tertiary Incubation

N/A

Detection

Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection

Results Summary

This antibody works very well on fixed tissue section of embryos. High specificity for epithelial microtubules, including primary cilia.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal for microtubules, especially in epithelia

The Bad

None known

The Bottom Line

Good antibody overall. Recommended for immunofluorescence study of epithelial-mesenchyme structures on tissue sections. This antibody reacts with acetylated alpha Tubulin, essentially in microtubule cytoskeleton and primary cilia

Share your experience with other scientists. Write a Review! »

Join the discussion