Sigma-Aldrich
Monoclonal Anti- Slow Myosin (Skeletal, Slow) antibody
M8421
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This antibody (clone NOQ7.5.4D) was used to analyze the presence of myotubes in differentiated culture of mouse embryonic stem cells (mESCs). This monoclonal anti- Slow Myosin Heavy chain (skeletal) antibody appears highly specific to differentiated muscle lineage, in particular myocytes and myotubes.
Immunofluorescence
Mouse embryonic stem cells (mESCs), differentiated. Fixed Adherent culture.
1:300, overnight, at 4C in Blocking agent, on shaker
TBS, 1% FBS, 0.1% TritonX100, 30min, at RT
1:500, AlexaFluor-488-conjugated anti-mouse IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on shaker
N/A
Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection
This antibody works well on fixed adherent culture containing myogenic cells. Specific to myocytes and myotubes.
Specific signal (myotubes and myocytes) by immunofluorescence
None known
Good antibody overall. Recommended for immunofluorescence study of skeletal myogenesis in mouse. This antibody reacts against the slow Myosin Heavy chain isoform.