GRP78 Expression In Melanoma Cells

Department of Oncology
University of Pennsylvania
Post Doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

GRP78

Catalog Number:

ab21685

To understand the role of stress responses that promote resistance to targeted therapies in melanoma.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human melanoma A375 cell lines

Primary Incubation

Overnight incubation at 4 C with 1:1000 dilution of GRP78

Blocking Agent

5% Skimmed milk in PBST for 2 hours

Secondary Incubation

1 hours with 1:5000

Tertiary Incubation

Not required

Detection

ECL

Results Summary

Very nice bands without no significant background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very strong band

The Bad

Nothing

The Bottom Line

Highly recommended forWestern, IP and immunofluorescence.

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