pAkt In Mouse Prostate

Cancer Centre
Cold Spring Harbor Laboratory
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

pAkt (Ser 473)

Catalog Number:

4060

The goal was to see if pAkt was present in the mouse prostate in metastatic disease.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse prostate

Primary Incubation

0.3% H2O2 in PBS

Blocking Agent

10% natural goat serum in PBS

Secondary Incubation

2nd biotin conjugated antibody diluted in PBS

Tertiary Incubation

Vectastain abc kit

Detection

Hematoxylin

Results Summary

Focal disease in the mouse prostate was shown using pAkt.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Publishable staining

The Bad

N/A

The Bottom Line

The Ab worked great, with reproducible results and publishable quality.

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