Thermo Scientific
SuperSignal West Dura Extended Duration Substrate
34075
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This DURA substrate is a good choice when you need to enhance the sensitivity or duration of the signal for your Western Blotting. The antibody concentration and the exposure time for imaging can be greatly reduced. I have been using this one for more than 6 years and it is better than many other similar products on the market.
Western Blotting
Any PVDF membrane
Easy and simple. Just mix the two reagents from the box and incubate your blot for few minutes (though the kit suggests 5 min, I found that 1-2 min is good enough in most of the situations)
Use lesser antibody or reduce the exposure time to obtain a better result
I always get a very strong signal of protein band in few seconds. Save a lot of time and the antibodies.
N/A
High sensitivity and longer duration
A little pricey
Use lesser antibody and shorter exposure time are critical to obtaining nice bands.
Product Name: SuperSignal West Dura Extended Duration Substrate, 100mL kitSupplier Page