Good Codon Optimization Service

Microbiology & Immunology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

GenScript

Service Provided:

Codon optimization and gene synthesis

Genscript performed codon optimization for several human TCR alpha and beta chain genes. We sent them the sequence of out original TCR chains (about 2kb total) and they did the rest. After analyzing our sequenced they optimized it for optimal expression and synthesized the genes and sent it to us in a pUC57 vector.

Experimental Design and Results Summary

Downstream Applications

Optimized sequences were used to generate lentivectors encoding the TCR genes to transduced human CD8 T cells

Timeline

2 weeks

Nature of Service

Codon optimization of several human TCR alpha and beta genes

Technical Service

Very responsive and helpful

Delivery

No issues

Results Summary

We sent GenScript the sequence of out original TCR chain genes (about 2kb total) and they did the rest. After analyzing our sequenced they optimized it for optimal expression and synthesized the genes and sent it to us in a pUC57 vector.

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Summary

The Good

Great technical support, helpful representatives

The Bad

A little expensive, could be a little faster

The Bottom Line

Great company, we have used their services many times for codon optimization.

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