Cell Signaling Technology
Phospho-FLT3 (Tyr591) Antibody
3461
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The general aim of my research is to study signaling pathways involved in the pathogenesis of leukemia by specifically studying signaling in acute myeloid leukemia (AML) stem cells. The specific goal of the experiment for which I used the product was to determine whether primary AML blood cells are able to be stimulated by various cytokine receptor ligands and to look at the downstream effects on signaling of stimulation with these ligands. The reason I selected this product is because I have had previous success with Cell Signaling phospho-antibodies.
Western Blot
Human primary acute myeloid leukemia blood mononuclear cells
1:1000, overnight at 4 degrees C with rocking
TBS + 0.1% Tween + 5% w/v BSA
1:5000 anti-rabbit-HRP, 1 hour at room temperature with rocking
None
ECL
Increased density of bands at ~110-120 kDa and ~130 kDa after stimulation with FLT3 ligand
Strong band at expected size
Some nonspecific bands around 70 kDa
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