GAPDH Antibody

Pulmonary disease
Tulane University
Post Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell signaling technology

Product Name:

GAPDH antibody

Catalog Number:

2118S

Our interest is to see the expression of fibrosis associated genes during chronic disorders. Therefore we need a loading control for western blot analysis hence I used this GAPDH monoclonal antibody as control. Very good antibody to detect the protein level of GAPDH protein from human, mouse and rat.

 

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse pancreas

Primary Incubation

1:1000

Blocking Agent

5% BSA

Secondary Incubation

Goat anti rabbit

Tertiary Incubation

NA

Detection

Luminiscence

Results Summary

This is a monoclonal antibody and detect a sharp single band of GAPDH at expected molecular weight.

Additional Notes

NA

Related Categories

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Summary

The Good

Antibody is very specific and there is no non specific detection.

The Bad

Do not reuse the antibody.

The Bottom Line

Overall this is very good antibody and it can be used as loading control for western blot.

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