1uL CD8-V450 Work Great To Detect CD8+ T Cells

Rheumatology
Vanderbilt University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

V450 Mouse Anti-Human CD8

Catalog Number:

560347

Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We use CD8-V450 in a few of our T cell panels because the staining looks great and it is cost-efficient.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

1uL CD8-V450 in 200uL PBS with 10^6 cells; 15 minutes; room temperature

Blocking Agent

None.

Secondary Incubation

None.

Tertiary Incubation

None.

Detection

BD LSRFortessa

Results Summary

CD8-V450 looks great using 1uL/sample. As evidenced in the attached image the staining pattern looks good and it works well in a multi-color panel.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Staining pattern is great; cost-efficient

The Bad

None.

The Bottom Line

BD CD8-V450 is a great option for detecting CD8+ T cells in multi-color flow panels.

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