2uL BD CD4-BV605 Works Great In Multi-Color Panel

Rheumatology
Vanderbilt University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BV605 Mouse Anti-Human CD4

Catalog Number:

562658

Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. Moving CD4 to a Brilliant Violet has given us more flexibility in our panels.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC

Primary Incubation

2uL CD4-BV605 in 200uL PBS with 10^6 cells; 15 minutes; room temperature

Blocking Agent

None.

Secondary Incubation

None.

Tertiary Incubation

None.

Detection

BD LSRFortessa

Results Summary

BD CD4-BV605 works great in a multi-color panel. As seen in the included image, 2uL works great to detect CD4+ T cells ex vivo. Additionally, it is sensitive enough to detect shifts in CD4 expression as with potent stimulation as evidenced by the shift in CD4 MFI with SEB stimulation (1ug/mL).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

2uL CD4-BV605 works great!

The Bad

Significant bleedover into the PETR channel.

The Bottom Line

BD CD4-BV605 yields a great staining pattern to detect CD4+ T cells in a multi-color panel.

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