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FITC anti-human CD54 Antibody
353107
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CD54 is a surface marker, involved in cellular adhesion, inflammation and leukocyte extravasation. It is upregulated activation of monocytes (and other cells). We used it to evaluate the success of the stimulation we applied to our monocytes.
Flow Cytometry
THP-1 human monocytic cell line
30 minutes (room temp)
Human IgG solution (Sigma)
None
Flow cytometer
The unstimulated cells (blue histogram) have little/no CD54, while those stimulated with 0.2uM PMA (red histogram) show big upregulation of CD54.
Easy to use, strong signal
A good antibody, at a good price!