eBioscience
Anti-Human/Mouse ROR gamma (t) PE
12-6988-82
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Browse Similar Products: ROR gamma Flow Cytometry Antibodies
Staining for Th17 cells or ILC3s? So were we, and we found this to be a great antibody to use. I wouldn't mind a different color other than PE (personal preference mostly), but this works well with our ex vivo and in vitro cells.
Flow Cytometry
Mouse cells ex vivo
40 minute incubation in Tonbo Perm buffer
None
Flow cytometry (488 laser, BD CantoII)
Staining of both in vitro cultured cells and ex vivo cell suspensions from spleen, mLN, and intestines work well. Amount of antibody used is 0.4ul per sample in 50ul of Tonbo perm buffer (after Tonbo Fix/Perm O/N).
Ease of use, primary antibody
PE signal isn't overly bright
Strongly recommend this antibody. Separation between positive and negative populations is good (especially for a transcription factor).