The Antibody Stains Sca1+ Cells Very Well

October 05, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmingen

Product Name:

FITC Rat Anti-Mouse Ly-6A/E

Catalog Number:

553335

We wanted to look at how Sca1+ cells changed in the normal vs tumor setting, so we used this antibody to identify the cells via flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse intestinal cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody stains Sca1+ cells very well.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody stains Sca1+ cells clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying Sca1+ cells in the intestine

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