CD11b Skin IF Staining

October 03, 2016

UIC
Research Scientist

Overall

Quality of Results

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Company:

Novusbio

Product Name:

CD11b antibody

Catalog Number:

NB110-89474SS

The aim is to detect CD11b positive CD68 + macrophages. This antibody had high background and seemed to not stain CD68+ macrophages.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse skin

Primary Incubation

45min RT

Blocking Agent

10% goat serum

Secondary Incubation

Goat anti rabbit Alexa Fluor 488

Tertiary Incubation

None

Detection

Alexa Fluor 488

Results Summary

This CD11b antibody seemed not to stain CD68+ macrophages (red) with high background (green).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

None

The Bad

High background/non-specific

The Bottom Line

Not recommended for IF mouse skin CD11b staining.

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