Abcam
Anti-Pericentrin Antibody
ab4448
Info: View Product View Product Specs
Browse Similar Products: Pericentrin Immunofluorescence Antibodies
Antibody is really really good and works incredibly well to mark centrosomes. Antibody is on the more expensive side, but antibody works well at 1:2000. This is the protocol used: Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. 3 minutes in ice-cold methanol. 5 minutes in PBS+0.5% Triton X5 minutes in PBS 10 minutes in PBS+3% BSA Antibody was incubated in PBS+1% BSA and coverslips incubated overnight at 4C2 - 5 minute washes in PBS Secondary antibodies from Jackson Laboratories (anti-rabbit) was incubated in PBS+1% BSA and coverslips 2 - 5 minute washes in PBS Mounted coverslips using ProLong Gold
Immunofluorescence
RPE-1 cells
1:2000 in PBS+1% BSA 1 hour room temp
PBS+3% BSA
1:1000 in PBS+1%BSA for 1 hour
None
Florescence imaging was done on a 60X objective
Antibody is very strong and specific. I recommend using Methanol fixation alone or paraformaldehyde + methanol (as was described in the protocol) to keep centrosomes in tact. Overall antibody worked very well for IF
Works great
On the expensive side
Great centrosome marker