Zeiss
PALM Laser Capture Microdissection System
PALM MicroBeam
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The overall goal is of this research is to phenotype early targets of HIV/SIV. The specific goal is to use laser capture microdisection approach to isolate transduced cells across different tissues. This system was chosen because of the availability within the core facility.
Microscopy, Lase Microdissections
Rhesus macaque frozen tissue
Tissues were cryosectioned and processed using Histogene Staining Kit by Arcturus. PALM MicroBeam lasers were adjusted for the strength and focus to get the tissue to cut cleanly (with minimal debre). Regions of the selected tissue were catapulted into a lid of a small tube that was immediately placed on ice and used for RNA extractions.
Hiting the right balance between laser strength and focus will need to be optimized each time
We were able to get very clean cut of the tissue so that single cell or group of cells could be excised and collected into the tube. However we had to optimize laser strength and focus to make sure optimal performance is achieved. Humidity, tissue thickness and tissue type all played role.
Able to adjust laser strength and focus, also able to perform immunogluorescence staining
None
Able to get single cells isolated
Lots of trial and error
Useful system to get specific cell populations
Product Name: ZEISS PALM Microbeam Laser Microdissection SystemSupplier Page