Perfect NCAM (CD56) Antibody

September 19, 2016

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

NCAM (CD56) (123C3) Mouse mAb

Catalog Number:

3576

This is a very good NCAM (CD56) antibody. Just follow the instructions of the manufacturer. I am interested in different proteins expressed on extracellular vesicles.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Patient-derived glioma cell lysates

Primary Incubation

5% BSA, 4°C, overnight, shaker

Blocking Agent

5% milk, 1h, RT, shaker,

Secondary Incubation

5% milk, 1h, RT, shaker,

Tertiary Incubation

N/A

Detection

BIO RAD Imager

Results Summary

Perfect NCAM antibody. I also used it for IF and it works just as good.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

easy to use

The Bad

none

The Bottom Line

Perfect NCAM (CD56) antibody for WB and IF, other applications not tested

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