BosterBio Sparcl1

Neuroscience
Tufts University
Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

BosterBio

Product Name:

Anti-SPARCL1

Catalog Number:

PA2088

The general aim of the experiment was to determine molecular differences of astrocytes in the central nervous system. We wanted to see if there are different expression levels of Sparcl1 in different regions of the mouse brain as a way to compare and possibly validate qPCR data.The product was found on One World Lab and it worked so we decided to buy a greater quantity as opposed to a trial size.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse astrocytes

Primary Incubation

1:50, 3 nights

Blocking Agent

BSA, NGS

Secondary Incubation

1:2000

Tertiary Incubation

None

Detection

Confocal/Fluorescent Scope

Results Summary

Good.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works

The Bad

Long primary incubation time

The Bottom Line

Good.

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