Anti-TrkA-FITC (Immunofluorescence)

September 22, 2016

Anesthesiology
University of Wisconsin-Madison
Research Associate

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Company:

Millipore

Product Name:

Anti-TrkA-FITC

Catalog Number:

FCABS350F

This antibody was used to detect primary dorsal root ganglion (DRG) neurons isolated from mice. TrkA was used as a marker to detect and isolate the peptidergic subclass of DRG neurons for subsequent downstream analysis. This antibody worked to detect a subpopulation of DRG neurons in primary cell culture 5 days post-isolation.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse primary DRG neurons cultured for 5 days post-isolation in neurobasal medium (ThermoFisher).

Primary Incubation

A dilution of 1:100 of anti-TrkA-FITC in neurobasal medium incubated for 1 1/2 hours at 37C.

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

FITC was detected using a 480nm excitation wavelength on an inverted microscope.

Results Summary

The anti-TrkA antibody worked to detect a subclass of DRG neurons in culture. The signal was weak, but higher than our negative control anti-rabbit-FITC staining.

Additional Notes

Data sheet reports epitope was of rat origin and that the antibody has specificity against human TrkA. It appears that it was successful in detecting mouse TrkA as well.

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Summary

The Good

Antibody worked, giving signal great than negative control staining.

The Bad

The signal was weak at relative high dilution.

The Bottom Line

I would cautiously recommend this antibody to other researchers given the low signal being detected above baseline.

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