Tonbo Biosciences
Ghost Dye™ Violet 450
13-0863-T500
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Amine reactive dye that works well with in vitro cultured cells (used mainly for primary lymphocytes). Very bright in the Pacific Blue channel, and I can easily use a quarter of the recommended volume to get bright staining. Staining in PBS is easy enough (10 minute incubation is sufficient). Very low crossover into FITC and PE channels.
Flow Cytometry
Cultured primary T lymphocytes
Ghost Dye™ Violet 450 in PBS
None
Flow cytometry (Violet laser, Pacific Blue channel; BD CantoII)
I picked this viability dye since I seldom use the Pacific Blue channel for my antibodies. I can maximize the number of channels open with this method, while avoiding compensation issues.
Bright and fast staining, low crossover in other common channels
Not the cheapest viability dye available
I'd recommend this dye for anyone looking to fill their Pacific Blue channel with a viability dye.