Biolegend
PE anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody
108407
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The purpose of experiment was to specifically label GR-1 marker for which this antibody was purchased. While labeling is specific, due to strong PE label antibody optimization is critically recommended. I went very high in diluting before getting specific signal.
Flow Cytometry
Mouse spleen cells
1:2000 in CD16/32 block
CD16/32 or Fc block
None
Not applicable
PE
Specific labeling was obtained however after antibody titration.
Strong signal
need to titrate antibody to very high dilutions
Overall its good antibody if a person is not lazy to titrate.