Cell Signaling Technology
EGF Receptor Antibody
2232
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The long-term goal of this project is to test the effect of EGFR inhibition on the proliferative properties of human primary keratinocytes. The first step in this project was to determine the expression of EGFR by Western Blotting. This antibody from Cell Signaling Technology was used because more than 20 papers have cited this product.
Western Blot
Human primary keratinocyte culture lysate
1:1000 in blocking buffer; 16 hr at 4 deg
5% BSA in 1xTBS-T(0.1%)
Goat-anti Rabbit-IgG-HRP conjugated; 1:10,000 dilution in blocking agent
None
ECL regular
Human primary keratinocytes express good amounts of EGFR as detected by Western Blotting. 50 µg of total cell lysate was sufficient to give a good signal.
Specific signal
Cell Signaling antibodies never disappoint!