DRG Diagnostics
Insulin (Rat) EIA
EIA-2048
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Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. The goal of this project is to understand the role of Protein Kinase D in the secretory pathway. In the present experiment, glucose stimulated insulin secretion was measured in INS-1E cells with a long-term goal to test the inhibition of Protein Kinase D on insulin secretion in these cells.
ELISA
INS-1E cell supernatant
100,000 or 200,000 cells (INS-1E cell line) was plated in a 24 well plate. Confluent cells were serum starved for 12 hr and then stimulated with either 2.5 or 20 mM glucose for 30 min. Supernatants were then collected for ELISA experiment.
Follow the instructions form the kit and it works very well
In INS-1E cells, 20 mM glucose treatment could nicely elicit insulin secretion.
None
Works very well
Works well but a bit expensive
Product Name: Insulin (rat)Supplier Page