BCA Protein Estimation Kit

Tulane University
Pulmonary disease
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Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Scientific

Product Name:

Pierce BCA protein estimation kit

Catalog Number:

23227

Kit works very nicely to determine the accurate protein concentration from either cell lysate or tissue homogenate.

Experimental Design and Results Summary

Application

Estimation of total protein concentration from cell and tissue lysate

Starting Material

Cell lysate or tissue lysate

Protocol Overview

Protocol is easily optimized

Tips

We use 96 well plate and reduced the volume of each reagent and protein 1/4 in order to save kit and protein.

Results Summary

Results of protein concentration depends on type of tissue or cell type.

Additional Notes

None

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Summary

The Good

Very little time is required to estimate protein concentration.

The Bad

N/A

The Bottom Line

Kit can be used to quick measurement of protein concentration that can be used further for ELISA, westerns, and 2D.

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