Abcam
Anti-Firefly Luciferase antibody
ab21176
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The general aim of our research is to gain insights into SIV/HIV transmission. Our specific goals are to investigate what are SIV/HIV target cells and where in the tissues virus can reach. This product was chosen because good relation with the company and price.
Western Blot
293T Cells
One hour at room temperature
Normal donkey serum
Donkey anti-rabbit antibody
None
When probing with Luciferase antibody there is a one strong band at 70 kDa (Luciferase alone), one fainter band at 110 kDa (most likely all three proteins together) and then some smear which seems to be like protein degradation.
Ease of use, good signal
Needs to be alliquoted
Very good antibody for Western Blot protein detection.