Great HLA-A2 Antibody

Microbiology/Immunology
Loyola University Health science campus
PhD student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Pharmigen

Product Name:

PE Mouse anti-Human HLA-A2

Catalog Number:

558570

I use this antibody to detect human HLA-A2 expression on murine melanoma cells transfected to express HLA-A2. In the past, other antibodies resulted in high background staining for our HLA-A2 negative melanoma variant. This antibody, however, was much cleaner and much better quality.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Melanoma cells

Primary Incubation

5ul

Blocking Agent

Fc gamma

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

No background staining on A2 negative tumor line. Great staining for our A2 positive tumor line

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

No background staining

The Bad

Nothing

The Bottom Line

Great antibody, Titrated the amount of antibody needed to about 5 ul per million cells

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