Good PLK1 (208G4) Rabbit mAb

August 25, 2016

Pathology
University of Kansas Medical Center
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technology

Product Name:

PLK1 (208G4) Rabbit mAb

Catalog Number:

4513S

Western blot assays to validate protein array data in ovarian cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Ovarian cancer cell line panel lysates

Primary Incubation

1:1000 in 5% milk in TBS-T, overnight at 4 degrees C, on a rocker

Blocking Agent

5% milk in TBS-T

Secondary Incubation

1:5000, anti-Rb in 5% milk in TBS-T, 30 minutes at room temp, on a rocker

Tertiary Incubation

None

Detection

HRP Chemiluminescence

Results Summary

Good antibody with nice clear bands at the correct molecular weight (60 kDa).

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clean bands, good antibody

The Bad

None

The Bottom Line

Good antibody, didn't require much optimization. Easy to use.

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