BD Biosciences
PerCP Rat Anti-Mouse CD8a
553036
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The objective of this experiment was to study T cell subsets in mouse spleens. I tested if the anti-CD8 PerCp antibody from BD can be used as an antibody to identify the CD8 positive populations. Single cell suspensions isolated from mouse splenocytes were stained with this antibody and analyzed by flow cytometry. The result demonstrates that I was able to separate CD8 positive cells from rest of the splenocytes.
Flow Cytometry
Mouse splenocytes
30
None
Flow cytometry in PerCp Channel
The results show a clear CD8 positive and negative population which confirm the specificity of the antibody
Specificity
Good anti-mouse CD8 PerCp antibody