Santa Cruz Biotechnology
anti-BDNF (H117)
sc-20981
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Anti-BDNF was employed to analyze its expression by western blotting in cortex tissue from STZ-injected animals.
Western Blot
Brain protein extract (Cortex)
1:500, Over night, 4˚C, incubated in 5% BSA in TBS-T
5% BSA in TBS-T
1:4000, 1 hour, room temperature, incubated in 5% BSA in TBS-T
Not necessary
Chemiluminescent detection
Sc-20981 worked fine at 1:1000 for 60ug of proteins brain extract. It recognizes different bands: 1) at ~14KDa (mature) and 2) at ~30KDa (immature)
None
Good results
Nothing
I was happy with the result