Biorbyt
FUNDC1 antibody
156964
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Browse Similar Products: FUNDC1 Western Blot Antibodies
Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.
Western Blot
Mouse ES cells
1:1000 4C overnight
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Not bad for screening knockouts, but there is a nonspecific band close to the expected band which complicates analysis. I think the lower band is the correct band, though I can't be sure.
None
Relatively cheap
Unexpected band
Probably wouldn't use this antibody again, would need another antibody to confirm analysis and conclusions.