Genetex Prdm6 Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Genetex

Product Name:

PRDM6 antibody

Catalog Number:

GTX32807

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Ok antibody, multiple bands, some background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear band at right size

The Bad

Additional nonspecific bands

The Bottom Line

Okay antibody for use in mouse ES cells, though there are probably better antibodies out there in the market.

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