EMD Millipore
Glyceraldehyde 3-phosphate dehydrogenase Antibody
CB1001
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Rat tissue panel analyzing the differential expression level of common endogenous loading control genes. EMD Millipore (#CB1001) anti-GAPDH immunoreactivity is shown below the beta-actin signal on the western blot.
Western Blot
Various rat tissues were homogenized and cellular proteins (20ug) were extracted in RIPA buffer were analyzed by western blot.
1:10,000 in 2% milk incubated at 4C overnight
5% milk
Anti-Mouse-HRP (1:5000) in 2% milk incubated for 1 hour at room temperature
N/A
ECL Western Blotting substrate (Promega, #W1001)
Antibody performed well and successfully detected endogenous rat GAPDH.
GAPDH levels vary among different rat tissues.
Antibody performed very well at relatively low dilutions and showed strong, clean signal.
None to report
I would recommend this antibody to investigators.