Sigma Aldrich
Anti-beta Actin
A1978
Browse Similar Products: Beta Actin Western Blot Antibodies
Rat tissue panel analyzing the differential expression level of common endogenous loading control genes. Sigma Aldrich (#A1978) anti-beta actin immunoreactivity is shown above GAPDH on the western blot.
Western Blot
Various rat tissues were homogenized and cellular proteins (20ug) were extracted in RIPA buffer were analyzed by western blot.
1:5000 in 2% milk and incubated at 4C, overnight
5% milk
Anti-Mouse-HRP (1:5000) in 2% milk incubated for 1 hour at room temperature
N/A
ECL Western Blotting substrate (Promega, #W1001)
Antibody performed well and successfully detected endogenous rat beta-actin.
Beta-actin levels vary among different rat tissues.
Antibody worked well and showed strong, clean signal.
None to report
I would recommend this antibody to investigators.