Anti-LMAN1 (Western Blot)

August 04, 2016

Anesthesiology
University of Wisconsin-Madison
Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam Inc.

Product Name:

Anti-LMAN1

Catalog Number:

ab125006

LMAN1 immunodectection of endogenous HepG2 LMAN1 protein in siRNA transfected cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Endogenous HepG2 lysate collected after siRNA transfection targeting LMAN1. Lane 1: Scrambled siRNA, Lane 2: LMAN1 siRNA.

Primary Incubation

1:1000 in 2% milk incubated at 4C, overnight

Blocking Agent

5% milk

Secondary Incubation

Anti-Rabbit-HRP (1:3000) in 2% milk incubated for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL Western Blotting substrate (Promega, #W1001)

Results Summary

Anti-LMAN1 (abcam #ab125006) worked well to detect endogenous HepG2 LMAN1. Signal gave good specificity as confirmed by siRNA knockdown.

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody worked well giving specific, sensitive signal.

The Bad

None to report

The Bottom Line

I would recommend this antibody to investigators.

Share your experience with other scientists. Write a Review! »

Join the discussion