Rabbit polyclonal antibody AP19833a against the N-terminus of EGFR from Abgent

August 02, 2016

The Cell and Molecular Biology
The Scripps Research Institute
Assistant Professor

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Company:

Abgent

Product Name:

EGRF Antibody (N-Term)

Catalog Number:

AP19833a

Rabbit polyclonal antibody AP19833a against the N-terminus of EGFR from Abgent was probed for western blot detection of the recombinant N-terminal domain of human EGFR (1-621 aa). The antibody nicely detected the protein band of ~100 kDa, consistent with the expected mol. wt. of the recombinant protein.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Recombinant N-Terminal domain of human EGFR (aa 1-621) tested at 100 ng/lane

Primary Incubation

Overnight, at 4-8 degrees Celsius, with rocking, at 1 ug/mL in 5% milk/PBS/0.05% Tween 20

Blocking Agent

5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)

Secondary Incubation

Goat anti-rabbit antibody conjugated with HRP at 1:5,000 in 5% milk/PBS/Tw

Tertiary Incubation

None

Detection

SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)

Results Summary

The rabbit antibody AP1933a nicely detected the recombinant N-terminal domain EGFR loaded at 100 ng/lane.

Additional Notes

None

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Summary

The Good

Antibody appears to be highly specific since no non-specific bands were detected in the parallel lanes with cell lysates lacking intact EGFR.

The Bad

None detected

The Bottom Line

The antibody AP19833a can be recommended for western blot detection of the N-terminus of human EGFR.

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